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collagen iv fibronectin coated plates  (Thermo Fisher)


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    Thermo Fisher collagen iv fibronectin coated plates
    Collagen Iv Fibronectin Coated Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/Collagen/10__1161_slash_atvbaha__125__323397-73-8-29
    Average 99 stars, based on 1 article reviews
    collagen iv fibronectin coated plates - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies
    Article Snippet: These endothelial cells, now termed bpECs, were plated on collagen IV/fibronectin coated plates at a density of 30,000 to 35,000 cells/cm and fed EC medium (Human Endothelial Serum Free Medium (Thermo Fisher) supplemented with 1% human serum (Sigma-Aldrich) supplemented with 50 ng/mL VEGF165 (R&D), 20 ng/mL bFGF (R&D), and 10 μM SB431542 (Sigma-Aldrich). bpECs were expanded and passaged 1:2 to 1:3 every 7-10 days and generally used for five passages.

    Article Title: Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional Reprogramming
    Article Snippet: These ECs, now termed bpECs, were plated on collagen IV/fibronectin-coated plates at a density of 30 000 to 35 000 cells/cm2 and fed EC medium (human endothelial serum-free medium [Thermo Fisher]) supplemented with 1% human serum (Sigma-Aldrich) supplemented with 50-ng/mL VEGF-A165 (R&D), 20-ng/mL bFGF (R&D), and 10-μM SB431542 (Sigma-Aldrich). bpECs were expanded and passaged 1:2 to 1:3 every 7 to 10 days and generally used for 5 passages.



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    Fig. 2 Characterization of receptor expression in two human cell barrier models. A Sorting receptors TfR and sortilin are both expressed in hiPSC-BECs and Caco-2 cells and localized at intracellular surfaces. The polarized receptors podocalyxin and CD133 are both expressed in the apical surface facing the luminal side of the Transwell chamber. B Micrographs showing maximum projected z-stacks with side view zx images above and xy images below. Cells are stained for ZO1 in white and the indicated receptors in green. Scale bars are 10 µm. See also Additional file 2: Fig S2

    Journal: Fluids and barriers of the CNS

    Article Title: Subcellular trafficking and transcytosis efficacy of different receptor types for therapeutic antibody delivery at the blood‒brain barrier.

    doi: 10.1186/s12987-023-00480-x

    Figure Lengend Snippet: Fig. 2 Characterization of receptor expression in two human cell barrier models. A Sorting receptors TfR and sortilin are both expressed in hiPSC-BECs and Caco-2 cells and localized at intracellular surfaces. The polarized receptors podocalyxin and CD133 are both expressed in the apical surface facing the luminal side of the Transwell chamber. B Micrographs showing maximum projected z-stacks with side view zx images above and xy images below. Cells are stained for ZO1 in white and the indicated receptors in green. Scale bars are 10 µm. See also Additional file 2: Fig S2

    Article Snippet: Then, 500,000 cells were seeded in EC medium onto collagen IV/fibronectin-coated Transwell thinserts (12- well format, 1.13 cm2, GREINER BIO-ONE, Cat. No.: GR-665641) with or without astrocyte noncontact coculture.

    Techniques: Expressing, Staining

    Fig. 4 Transcytosis of cargo antibodies through cell barriers. A ELISA measurement of cargo antibody in the Transwell bottom acceptor chamber in relation to the added antibody in the top donor chamber given as transcytosed IgG percentage through the hiPSC-BEC layer. The red stippled line indicates the lower level of detection (approx. 50 pM) for the ELISA setup. B ELISA quantification of cargo antibody transcytosis through the Caco-2 cell layer. Statistical significance was determined using two-way ANOVA with Tukey´s multiple comparisons test based on four independent experiments. See also Additional file 2: Fig S5

    Journal: Fluids and barriers of the CNS

    Article Title: Subcellular trafficking and transcytosis efficacy of different receptor types for therapeutic antibody delivery at the blood‒brain barrier.

    doi: 10.1186/s12987-023-00480-x

    Figure Lengend Snippet: Fig. 4 Transcytosis of cargo antibodies through cell barriers. A ELISA measurement of cargo antibody in the Transwell bottom acceptor chamber in relation to the added antibody in the top donor chamber given as transcytosed IgG percentage through the hiPSC-BEC layer. The red stippled line indicates the lower level of detection (approx. 50 pM) for the ELISA setup. B ELISA quantification of cargo antibody transcytosis through the Caco-2 cell layer. Statistical significance was determined using two-way ANOVA with Tukey´s multiple comparisons test based on four independent experiments. See also Additional file 2: Fig S5

    Article Snippet: Then, 500,000 cells were seeded in EC medium onto collagen IV/fibronectin-coated Transwell thinserts (12- well format, 1.13 cm2, GREINER BIO-ONE, Cat. No.: GR-665641) with or without astrocyte noncontact coculture.

    Techniques: Enzyme-linked Immunosorbent Assay